Journal:

Article Title: Functional cleavage of the common cytokine receptor ? chain (? c ) by calpain

doi:

Figure Lengend Snippet: Cytokine receptor subunits with PEST sequences in their cytoplasmic domains

Article Snippet: Immunoprecipitations were performed with immobilized anti-chicken IgY (Promega) and either chicken anti-human γ c antibody (Promega) or chicken IgY (H+Y) as a control antibody (Promega).

Techniques: Sequencing

Journal:

Article Title: Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus

doi: 10.1128/JVI.76.24.12803-12812.2002

Figure Lengend Snippet: Immunoblot analysis of CD40 in whole-cell lysates. Cell monolayers were infected with HCMV TB40E at an MOI of 1 (inf.) or mock infected (noninfected [n.i.]). Cell lysates were prepared at the indicated times and analyzed by immunoblotting. (A) CD40 protein levels were detected with a polyclonal rabbit serum directed against human CD40. (B) Blots from panel A were stripped and reprobed with a rabbit serum directed against calreticulin. (C) Signal intensities from panels A and B were densitometrically quantified; data represent the ratio of CD40 to calreticulin (y axis) versus time p.i. (x axis). (D) Protein lysates from infected or noninfected cells were treated with N-glycosidase F (+) or left untreated (−) prior to immunoblot analysis with anti-CD40 antibodies. Molecular masses are given in kilodaltons.

Article Snippet: The following monoclonal antibodies or polyclonal sera were used: mouse IgG1-fluorescein isothiocyanate (FITC)-conjugated anti-human CD54 (Calbiochem, La Jolla, Calif.), anti-human major histocompatibility complex class I (MHC-I) (BD PharMingen, San Diego, Calif.), mouse IgG1-FITC anti-human CD40 (Dianova, Hamburg, Germany), mouse IgG1-FITC anti-human CD62E (Calbiochem), antibodies G28.5 and Ro1 (anti-human CD40) (gifts from H. Engelmann, Institute for Immunology, University of Munich, Munich, Germany) ( 39 ), polyclonal rabbit anti-CD40 serum (Santa Cruz Biotechnology), rabbit antiserum against human calreticulin (Dianova), biotin-conjugated rabbit anti-mouse antibodies, and biotinylated swine anti-rabbit antibodies (Dako, Hamburg, Germany).

Techniques: Western Blot, Infection

Journal: The Journal of Cell Biology

Article Title: Ectodomain shedding of L1 adhesion molecule promotes cell migration by autocrine binding to integrins

doi: 10.1083/jcb.200101099

Figure Lengend Snippet: L1 is shed without cytoplasmic tail. (A) CHO or L1-transfected CHO cells were incubated at 37°C in suspension in medium without FCS for 1 h. The cell pellet was lysed and analyzed by Western blot with mAb UJ127.11 to the ectodomain or pcytL1 to the cytoplasmic domain of L1. (B) Schematic representation of L1 cleavage fragments and their relative localization. The location of antibody recognition sites are indicated. (1), mAb 5G3; (2), mAb UJ 127.11; (3) pcytL1.

Article Snippet: The mAb to αvβ5 (P1F6) was obtained from Chemicon, the mAb to human CD49e (SAM-1) was from Dianova, the human L1 mAb 5G3 was obtained from Becton Dickinson, and the mAb to CD49f (GoH3) was obtained from Beckman Coulter.

Techniques: Transfection, Incubation, Suspension, Western Blot